An Intersubunit Interaction between S4-S5 Linker and S6 Is Responsible for the Slow Off-gating Component in Shaker K+ Channels

• DOI: 10.1074/jbc.m109.097717
• Publication Date: 2010-03-05T01:18:52Z
• Authors: Zarah Batulan; Georges A. Haddad; Rikard Blunck
• Keywords: Shaker

Voltage-gated ion channels are controlled by the membrane potential, which is sensed peripheral, positively charged voltage sensors. The movement of residues in sensor may be detected as gating currents. In Shaker K(+) channels, currents asymmetric; although on-gating fast, off-gating contain a slow component. This component caused stabilization activated state and has been suggested to linked permeation or C-type inactivation. molecular determinants responsible for stabilization, however, remain unknown. Here, we identified an interaction between Arg-394, Glu-395, Leu-398 on C termini S4-S5 linker Tyr-485 S6 neighboring subunit, development Mutation involved this intersubunit modulated strength associated interaction. Impairment still led pore opening but did not exhibit kinetics. Development occurs under physiological conduction correlated with opening. We, thus, suggest that above stabilize channel open state.