miR-200a Regulates Epithelial-Mesenchymal to Stem-like Transition via ZEB2 and β-Catenin Signaling

• DOI: 10.1074/jbc.m110.133744
• Publication Date: 2010-09-08T01:09:36Z
• Authors: Hongping Xia; William K.C. Cheung; Johnny Sze; Gang Lu; Songshan Jiang; Hong Yao; Xiu-Wu Bian; Wai Sang Poon; Hsiang-fu Kung; Marie C. Lin
• Keywords: Mesenchyme

The emerging concept of generating cancer stem cells from epithelial-mesenchymal transition has attracted great interest; however, the factors and molecular mechanisms that govern this putative tumor-initiating process remain largely elusive. We report here miR-200a not only regulates but also stem-like in nasopharyngeal carcinoma cells. first showed stable knockdown promotes epithelium-like CNE-1 to mesenchymal phenotype. More importantly, it induced several cell-like traits, including CD133(+) side population, sphere formation capacity, vivo tumorigenicity nude mice, cell marker expression. Consistently, overexpression switched mesenchyme-like C666-1 epithelial state, accompanied by a significant reduction features. Furthermore, vitro differentiation tumor resulted diminished population induction. To investigate mechanism, we demonstrated controls targeting ZEB2, although differentially specifically β-catenin signaling. Our findings reveal for time function shifting states via reversible coined as through differential specific mechanisms.