Hepatic Overexpression of a Constitutively Active Form of Liver Glycogen Synthase Improves Glucose Homeostasis

• DOI: 10.1074/jbc.m110.157396
• Publication Date: 2010-09-15T01:17:13Z
• Authors: Susana Ros; Delia Zafra; Jordi Valles-Ortega; Mar García-Rocha; Stephen Forrow; Jorge Domínguez; Joaquim Calbó; Joan J. Guinovart
• Keywords: Glycogenesis; Glycogen branching enzyme; Glycogenolysis; Homeostasis; Glycogen debranching enzyme

In this study, we tested the efficacy of increasing liver glycogen synthase to improve blood glucose homeostasis. The overexpression wild-type in rats had no effect on homeostasis either fed or fasted state. contrast, expression a constitutively active mutant form enzyme caused significant lowering former but not latter Moreover, it markedly enhanced clearance when were challenged with load. Hepatic stores overexpressing activated state and response an oral load showed net decline during fasting. order test whether these effects maintained long term activation synthase, generated liver-specific transgenic mice expressing LGS form. These also capacity store improved tolerance Thus, conclude that improves without compromising glycogenolysis postabsorptive On basis findings, propose may provide potential strategy for improvement postprandial