A Systems Biology Approach for the Investigation of the Heparin/Heparan Sulfate Interactome

• DOI: 10.1074/jbc.m111.228114
• Publication Date: 2011-03-31T00:18:08Z
• Authors: Alessandro Ori; Mark C. Wilkinson; David G. Fernig
• Keywords: Interactome; Proteome; Human proteome project

A large body of evidence supports the involvement heparan sulfate (HS) proteoglycans in physiological processes such as development and diseases including cancer neurodegenerative disorders. The role HS emerges from its ability to interact regulate activity a vast number extracellular proteins growth factors matrix components. global view on how protein-HS interactions influence proteome and, consequently, cell function is currently lacking. Here, we systematically investigate functional structural properties that characterize HS-interacting network they form. We collected 435 human interacting with or structurally related heparin by integrating literature-derived affinity proteomics data. used this data set identify topological features distinguish heparin/HS-interacting rest analyze enrichment gene ontology terms, pathways, domain families heparin/HS-binding proteins. Our analysis revealed form highly interconnected network, which functionally linked pathological are characteristic higher organisms. Therefore, then investigated existence correlation between expansion heparin/HS interactome increase biological complexity metazoan lineage. strong positive biosynthetic machinery organism emerged. evolutionary was reinforced presence rudimentary unicellular at root