Conserved Overlapping Gene Arrangement, Restricted Expression, and Biochemical Activities of DNA Polymerase ν (POLN)

• DOI: 10.1074/jbc.m115.677419
• Publication Date: 2015-08-12T23:58:46Z
• Authors: Kei-ichi Takata; Junya Tomida; Shelley Reh; Lisa M. Swanhart; Minoru Takata; Neil A. Hukriede; Richard D. Wood
• Keywords: Morpholino

DNA polymerase ν (POLN) is one of 16 polymerases encoded in vertebrate genomes. It important to determine its gene expression patterns, biological roles, and biochemical activities. By quantitative analysis mRNA expression, we found that POLN from the zebrafish Danio rerio expressed predominantly testis. not detectably embryos or mouse embryonic stem cells. Consistent with this, injection POLN-specific morpholino antisense oligonucleotides did interfere development. Analysis transcripts revealed has an unusual arrangement, sharing a first exon HAUS3, encoding augmin-like complex subunit 3. HAUS3 broadly adult tissues, contrast POLN. Differential appears arise by alternate splicing mammalian cells zebrafish. When was ectopically overexpressed human cells, it specifically coimmunoprecipitated homologous recombination factors BRCA1 FANCJ, but previously suggested interaction partners (HELQ members Fanconi anemia core complex). Purified protein capable thymine glycol bypass strand displacement, activity dependent on basic amino acid residue known stabilize primer-template. These properties are conserved enzyme. Although physiological function pol remains be clarified, this study uncovers distinctive aspects control evolutionarily polymerase.