AP2 Clathrin Adaptor Complex, but Not AP1, Controls the Access of the Major Histocompatibility Complex (MHC) Class II to Endosomes

• DOI: 10.1074/jbc.m501357200
• Publication Date: 2005-03-05T01:12:56Z
• Authors: Marc Dugast; Hélène Toussaint; Christelle Dousset; Philippe Benaroch
• Keywords: AP-1 transcription factor

Newly synthesized MHC II alpha- and beta-chains associated with the invariant chain chaperone (Ii) enter endocytic pathway for Ii degradation loading peptides before transport to cell surface. It is unclear how alphabetaIi complexes are sorted from Golgi apparatus directed endosomes. However, indirect evidence tends support direct involving AP1 clathrin adaptor complex. Surprisingly, we show here that knocking down production of by RNA interference did not affect trafficking complexes. In contrast, AP2 depletion led a large increase in surface levels complexes, inhibited their rapid internalization, strongly delayed appearance mature intracellular compartments. Thus, systems studied here, internalization via an AP2-dependent represents key step delivery endosomes lysosomes.