Multiple and Additive Functions of ALDH3A1 and ALDH1A1
Mice, Knockout
0301 basic medicine
Aging
Proteasome Endopeptidase Complex
Ultraviolet Rays
Retinal Dehydrogenase
Aldehyde Dehydrogenase
Glutathione
Aldehyde Dehydrogenase 1 Family
Cataract
Cornea
Mice
Oxidative Stress
03 medical and health sciences
Phenotype
Lens, Crystalline
Animals
Eye Proteins
Oxidation-Reduction
DOI:
10.1074/jbc.m702076200
Publication Date:
2007-06-14T06:34:59Z
AUTHORS (11)
ABSTRACT
ALDH3A1 (aldehyde dehydrogenase 3A1) is abundant in the mouse cornea but undetectable lens, and ALDH1A1 present at lower (catalytic) levels lens. To test hypothesis that protect anterior segment of eye against environmentally induced oxidative damage, Aldh1a1(-/-)/Aldh3a1(-/-) double knock-out Aldh1a1(-/-) Aldh3a1(-/-) single mice were evaluated for biochemical changes cataract formation (lens opacification). The Aldh1a1/Aldh3a1- Aldh3a1-null develop cataracts posterior subcapsular regions as well punctate opacities cortex by 1 month age. Aldh1a1-null also later life (6-9 months age). One- to three-month-old Aldh-null exposed UVB exhibited accelerated lens opacification, which was more pronounced Aldh3a1(-/-)/Aldh1a1(-/-) compared with wild type animals. Cataract associated decreased proteasomal activity, increased protein oxidation, GSH levels, 4-hydroxy-2-nonenal- malondialdehyde-protein adducts. In conclusion, these findings support corneal via nonenzymatic (light filtering) enzymatic (detoxification) functions.
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