Comparative Analysis of Selenocysteine Machinery and Selenoproteome Gene Expression in Mouse Brain Identifies Neurons as Key Functional Sites of Selenium in Mammals

• DOI: 10.1074/jbc.m707951200
• Publication Date: 2007-11-22T01:15:08Z
• Authors: Yan Zhang; You Zhou; Ulrich Schweizer; Nicolai E. Savaskan; Deame Hua; Jonathan Kipnis; Dolph L. Hatfield; Vadim N. Gladyshev
• Keywords: Selenoprotein; Selenocysteine

Although dietary selenium (Se) deficiency results in phenotypes associated with selenoprotein depletion various organs, the brain is protected from Se loss. To address basis for critical role of function, we carried out comparative gene expression analyses complete selenoproteome and biosynthetic factors. Using Allen Brain Atlas, evaluated 159 regions adult mouse provided experimental selected selenoproteins. All 24 mRNAs were expressed brain. Most strikingly, neurons olfactory bulb, hippocampus, cerebral cortex, cerebellar cortex exceptionally rich expression, particular GPx4, SelK, SelM, SelW, Sep15. Over half genes also choroid plexus. A unique pattern was observed one highly genes, SelP, which suggest to provide Se. Cluster analysis data linked certain selenoproteins selenocysteine machinery suggested functional linkages among selenoproteins, such as that between SelM Overall, this study suggests main functions mammals are confined