Intersectin Regulates Dendritic Spine Development and Somatodendritic Endocytosis but Not Synaptic Vesicle Recycling in Hippocampal Neurons

• DOI: 10.1074/jbc.m809746200
• Publication Date: 2009-03-04T02:14:22Z
• Authors: Sébastien Thomas; Brigitte Ritter; David Verbich; Claire Sanson; Lyne Bourbonnière; R. Anne McKinney; Peter S. McPherson
• Keywords: Synaptic vesicle recycling

Intersectin-short (intersectin-s) is a multimodule scaffolding protein functioning in constitutive and regulated forms of endocytosis non-neuronal cells synaptic vesicle (SV) recycling at the neuromuscular junction Drosophila Caenorhabditis elegans. In vertebrates, alternative splicing generates second isoform, intersectin-long (intersectin-l), that contains additional modular domains providing guanine nucleotide exchange factor activity for Cdc42. mammals, intersectin-s expressed multiple tissues cells, including glia, but excluded from neurons, whereas intersectin-l neuron-specific isoform. Thus, intersectin-I may regulate mammalian SV endocytosis. We now report, however, localized to somatodendritic regions cultured hippocampal with some juxtanuclear accumulation, synaptophysin-labeled axon terminals. Consistently, knockdown (KD) does not affect recycling. Instead co-localizes clathrin heavy chain adaptor 2 region its KD reduces rate transferrin The also F-actin dendritic spines, disrupts spine maturation during development. Our data indicate indeed an important regulator neuronal development it prominent player SVs.