Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

• DOI: 10.1074/mcp.m110.000109
• Publication Date: 2010-09-22T02:28:02Z
• Authors: Kimberly M. Wegener; Abhay K. Singh; Jon M. Jacobs; Thanura Elvitigala; Eric A. Welsh; Nir Keren; Marina A. Gritsenko; Bijoy K. Ghosh; David G. Camp; Richard D. Smith; Himadri B. Pakrasi
• Keywords: Proteome; Synechocystis; Assimilation (phonology); Nitrogen Cycle

Cyanobacteria, the only prokaryotes capable of oxygenic photosynthesis, are present in diverse ecological niches and play crucial roles global carbon nitrogen cycles. To proliferate nature, cyanobacteria utilize a host stress responses to accommodate periodic changes environmental conditions. A detailed knowledge composition of, as well dynamic in, proteome is necessary gain fundamental insights into such responses. Toward this goal, we have performed large-scale proteomic analysis widely studied model cyanobacterium Synechocystis sp. PCC 6803 under 33 different The resulting high-quality dataset consists 22,318 unique peptides corresponding 1955 proteins, coverage 53% predicted proteome. Quantitative determination protein abundances has led identification 1198 differentially regulated proteins. Notably, our revealed that common response various perturbations, irrespective amplitude duration, activation atypical pathways for acquisition from urea arginine. In particular, arginine catabolized via putrescine produce succinate glutamate, sources nitrogen, respectively. This study provides most comprehensive functional quantitative date, shows significant involves an uncommon mode nitrogen.