Characterization of Macaque Pulmonary Fluid Proteome during Monkeypox Infection

• DOI: 10.1074/mcp.m110.001875
• Publication Date: 2010-08-25T00:13:48Z
• Authors: Joseph N. Brown; Ryan D. Estep; Daniel Lopez-Ferrer; Heather M. Brewer; Theresa R. Clauss; Nathan P. Manes; Megan O'Connor; Helen Li; Joshua N. Adkins; Scott W. Wong; Richard D. Smith
• Keywords: Monkeypox; Proteome; S100A8; Neutrophil Extracellular Traps

Understanding viral pathogenesis is challenging because of confounding factors, including nonabrasive access to infected tissues and high abundance inflammatory mediators that may mask mechanistic details. In diseases such as influenza smallpox where the primary cause mortality results from complications in lung, characterization lung fluid offers a unique opportunity study host-pathogen interactions with minimal effect on animals. This investigation characterizes global proteome response pulmonary fluid, bronchoalveolar lavage macaques during upper respiratory infection by monkeypox virus (MPXV), close relative causative agent smallpox, variola virus. These are compared contrasted against infections vaccinia (VV), low pathogenic MPXV, extracellular MPXV-infected HeLa cells. To identify changes protein compartment, macaque was sampled twice prior infection, serving base line, up six times following intrabronchial either MPXV or VV. Increased expression proteins observed both viruses. Although increased resolved for subset proteins, C-reactive protein, S100A8, S100A9, levels persisted other vitamin D-binding fibrinogen γ. Structural metabolic were substantially decreased exclusively not VV infection. Decreases structural similarly Results this suggest host be only facilitator pathogenesis, but rather maintaining integrity could key factor influencing disease progression mortality.