Proteomic Analysis of Human Saliva From Lung Cancer Patients Using Two-Dimensional Difference Gel Electrophoresis and Mass Spectrometry

• DOI: 10.1074/mcp.m111.012112
• Publication Date: 2011-11-18T05:50:08Z
• Authors: Hua Xiao; Lei Zhang; Hui Zhou; Jay M. Lee; Edward B. Garon; David T.W. Wong
• Keywords: Aged, 80 and over; Male; 0303 health sciences; Lung Neoplasms; Proteome; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Middle Aged; Small Cell Lung Carcinoma; 3. Good health; Two-Dimensional Difference Gel Electrophoresis; 03 medical and health sciences; Carcinoma, Non-Small-Cell Lung; Case-Control Studies; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Biomarkers, Tumor; Humans; Female; Saliva; Lung; Cells, Cultured; Early Detection of Cancer; Aged

Lung cancer is often asymptomatic or causes only nonspecific symptoms in its early stages. Early detection represents one of the most promising approaches to reduce growing lung burden. Human saliva an attractive diagnostic fluid because collection less invasive than that tissue blood. Profiling proteins over course disease progression could reveal potential biomarkers indicative oral systematic diseases, which may be used extensively future medical diagnostics. There were 72 subjects enrolled this study for sample according approved protocol. Two-dimensional difference gel electrophoresis combined with MS was platform salivary proteome separation, quantification, and identification from two pooled samples. Candidate proteomic verified prevalidated by using immunoassay methods. 16 candidate protein discovered two-dimensional MS. Three further discovery set, prevalidation cell lines. The discriminatory power these patients healthy control can reach 88.5% sensitivity 92.3% specificity AUC = 0.90. This preliminary data report demonstrates are present human when people develop cancer. indicate a simple test might established clinical screening detection.