Coordinated Post-translational Responses of Aquaporins to Abiotic and Nutritional Stimuli in Arabidopsis Roots

• DOI: 10.1074/mcp.m113.028241
• Publication Date: 2013-09-21T06:31:35Z
• Authors: Magali di Pietro; Jérôme Vialaret; Guo-Wei Li; Sonia Hem; Karine Prado; Michel Rossignol; Christophe Maurel; Véronique Santoni
• Keywords: Starvation response

In plants, aquaporins play a crucial role in regulating root water transport response to environmental and physiological cues. Controls achieved at the post-translational level are thought be of critical importance for aquaporin function. To investigate general molecular mechanisms involved, we performed, using model species Arabidopsis, comprehensive proteomic analysis large set contexts. We identified nine treatments that modulate hydraulics time frames minutes (NO H2O2 treatments), hours (mannitol NaCl treatments, exposure darkness reversal with sucrose, phosphate supply phosphate-starved roots), or days (phosphate nitrogen starvation). All induced inhibition except sucrose dark-grown plants resupply which had opposing effects. Using robust label-free quantitative methodology, 12 13 plasma membrane intrinsic protein (PIP) isoforms, 4 10 tonoplast diversity modifications including phosphorylation, methylation, deamidation, acetylation. A total 55 peptides displayed significant changes after enabled identification specific as yet unknown patterns stimuli. The data show regulation PIP abundance was involved few (i.e. NaCl, NO, nitrate starvation), whereas phosphorylation status were positively correlated hydraulic conductivity whole treatments. vivo deamidated forms their stimulus-induced may reflect new mechanism regulation. overall work provides deep insights into events triggered by constraints possible plant status.