Hippocampal Extracellular Matrix Levels and Stochasticity in Synaptic Protein Expression Increase with Age and Are Associated with Age-dependent Cognitive Decline

• DOI: 10.1074/mcp.m113.032086
• Publication Date: 2014-07-21T00:10:59Z
• Authors: Marlene J. Végh; Antonio Rausell; Maarten Loos; Céline M. Heldring; Wiktor Jurkowski; Pim van Nierop; Iryna Paliukhovich; Ka Wan Li; Antonio del Sol; August B. Smit; Sabine Spijker; Ronald E. van Kes...
• Keywords: Cognitive Decline; Proteome

Age-related cognitive decline is a serious health concern in our aging society. Decreased function observed during healthy brain most likely caused by changes connectivity and synaptic dysfunction particular regions. Here we show that aged C57BL/6J wild-type mice have hippocampus-dependent spatial memory impairments. To identify the molecular mechanisms are relevant to these deficits, investigated temporal profile of mouse hippocampal proteome at 20, 40, 50, 60, 70, 80, 90, 100 weeks age. Extracellular matrix proteins were only group showed robust progressive up-regulation over time. This was confirmed immunoblotting histochemical analysis, which indicated increased levels extracellular might limit plasticity as potential cause age-related decline. In addition, stochasticity protein expression with age, for previously linked various neurodegenerative diseases, whereas low variance play basal role neuronal neurotransmission. Together, findings both specific associated may underlie reduced impaired performance old