Quantitative Mass Spectrometry Reveals Plasticity of Metabolic Networks in Mycobacterium smegmatis

• DOI: 10.1074/mcp.m113.034082
• Publication Date: 2014-07-06T00:18:38Z
• Authors: Tarun Chopra; Romain Hamelin; Florence Armand; Diego Chiappe; Marc Moniatte; John D. McKinney
• Keywords: Mycobacterium smegmatis; Proteome; Metabolic pathway; Acetyl-CoA; Catabolism

Mycobacterium tuberculosis has a remarkable ability to persist within the human host as clinically inapparent or chronically active infection. Fatty acids are thought be an important carbon source used by bacteria during long term Catabolism of fatty requires reprogramming metabolic networks, and enzymes central this have been targeted for drug discovery. smegmatis, nonpathogenic relative M. tuberculosis, is often model system because similarity basic cellular processes in these two species. Here, we take quantitative proteomics-based approach achieve global view how smegmatis network adjusts utilization source. Two-dimensional liquid chromatography mass spectrometry isotopically labeled proteins identified total 3,067 with high confidence. This number corresponds 44% predicted proteome includes most enzymes. Compared glucose-grown cells, 162 showed differential abundance acetate- propionate-grown cells. Among these, acetate-grown cells higher that could constitute functional glycerate pathway. Gene inactivation experiments confirmed both glyoxylate shunt pathway operational smegmatis. In addition annotated functions, demonstrate source-dependent not functionally characterized. These might play as-yet-unidentified roles mycobacterial metabolism. study reveals several novel features assimilation which suggests significant plasticity networks organism.