A Chemical Proteomics Approach for Global Analysis of Lysine Monomethylome Profiling *

• DOI: 10.1074/mcp.m114.044255
• Publication Date: 2014-12-12T05:42:06Z
• Authors: Zhixiang Wu; Zhongyi Cheng; Mingwei Sun; Xuelian Wan; Ping Liu; Tieming He; Minjia Tan; Yingming Zhao
• Keywords: Profiling (computer programming)

Methylation of lysine residues on histone proteins is known to play an important role in chromatin structure and function. However, non-histone protein substrates this modification remain largely unknown. An effective approach for system-wide analysis methylation, particularly monomethylation, lacking. Here we describe a chemical proteomics global screening monomethyllysine substrates, involving propionylation monomethylated lysine, affinity enrichment the modified peptides, HPLC/MS/MS analysis. Using approach, identified with high confidence 446 monomethylation sites 398 proteins, including three previously unknown marks, representing largest data set described date. Our not only confirms discovered methylation nucleus spliceosome, but also reveals new associated diverse biological processes. This method hence offers powerful dynamic study under cellular conditions human diseases.