Tracing Cellular Origin of Human Exosomes Using Multiplex Proximity Extension Assays

• DOI: 10.1074/mcp.m116.064725
• Publication Date: 2017-01-23T01:10:18Z
• Authors: Pia Larssen; Lotta Wik; Paulo Czarnewski; Maria Eldh; Liza Löf; K. Göran Ronquist; Louise Dubois; Eva Freyhult; Caroline J. Gallant; Johan Oelrich; Anders Larsson; Gunnar Ronquist; Eduardo J. Villab...; Ulf Landegren; Susanne Gabrielsson; Masood Kamali-Mog...
• Keywords: Multiplex; Tracing

Extracellular vesicles (EVs) are membrane-coated objects such as exosomes and microvesicles, released by many cell-types. Their presence in body fluids the variable surface composition content render them attractive potential biomarkers. The ability to determine their cellular origin could greatly move field forward. We used multiplex proximity extension assays (PEA) identify with high specificity sensitivity protein profiles of different origins, including seven cell lines two fluids. By comparing cells exosomes, we successfully identified originating exosomes. Furthermore, principal component analysis patterns human milk EVs prostasomes from prostate acinar clustered breast tissues, respectively. Milk uniquely expressed CXCL5, MIA, KLK6, whereas carried NKX31, GSTP1, SRC, highlighting that origins express distinct proteins. In conclusion, PEA provides a powerful screening tool exosome research, for purposes identifying source or new biomarkers diseases cancer inflammation.