Comparative Proteomic Analysis of Intra- and Interindividual Variation in Human Cerebrospinal Fluid

• DOI: 10.1074/mcp.m500207-mcp200
• Publication Date: 2005-10-02T00:12:42Z
• Authors: Yan Hu; James P. Malone; Anne M. Fagan; R. Reid Townsend; David M. Holtzman
• Keywords: Variation (astronomy)

Cerebrospinal fluid (CSF) is a potential source of biomarkers for many disorders the central nervous system, including Alzheimer disease (AD). Prior to comparing CSF samples between individuals identify patterns disease-associated proteins, it important examine variation within over short period time so that one can better interpret changes in as well given individual longer span. In this study, we analyzed 12 samples, composed pairs from six individuals, obtained 2 weeks apart. Multiaffinity depletion, two-dimensional DIGE, and tandem mass spectrometry were used. A number proteins whose abundance varied two points was identified each individual. Some these commonly multiple individuals. More importantly, despite intraindividual variations, hierarchical clustering multidimensional scaling analysis proteomic profiles revealed same cluster closest together between-subject variability much larger than within-subject variability. Among subjects, comparison four cognitively normal very mildly demented subjects also yielded some have been previous AD biomarker studies. These results validate our method identifying differences implications design studies other system disorders.