Characterization of an RNA Granule from Developing Brain

• DOI: 10.1074/mcp.m500255-mcp200
• Publication Date: 2005-12-13T01:32:59Z
• Authors: George Elvira; Sylwia Wasiak; Vanessa Blandford; Xin-Kang Tong; Alexandre Serrano; Xiaotang Fan; Maria del Rayo Sá...; Florence Servant; Alexander W. Bell; Daniel Boismenu; Jean-Claude Lacaille; Peter S. McPherson; Luc DesGroseillers; Wayne S. Sossin
• Keywords: Granule (geology)

In brain, mRNAs are transported from the cell body to processes, allowing for local protein translation at sites distant nucleus. Using subcellular fractionation, we isolated a fraction rat embryonic day 18 brains enriched structures that resemble amorphous collections of ribosomes. This was mRNA encoding beta-actin, an is in dendrites and axons developing neurons. Abundant components this fraction, determined by tandem mass spectrometry, include ribosomal proteins, RNA-binding microtubule-associated proteins (including motor dynein), several described only as potential open reading frames. The conjunction mRNA, machinery, transporting defines these RNA granules. Expression subset identified cultured hippocampal neurons confirmed proteomics were present neurites associated with ribosomes mRNAs. Moreover many expressed co-localized together. Time lapse video microscopy indicated complexes containing one DEAD box 3 helicase, migrated same speed reported Although granules unchanged activity or neurotrophin brain-derived neurotrophic factor, but not activity, increased proportion moving These studies define isolation composition brain.