Proteome Analysis of Human Hair Shaft

• DOI: 10.1074/mcp.m500278-mcp200
• Publication Date: 2006-01-31T01:23:49Z
• Authors: Young Jin Lee; Robert H. Rice; Young Moo Lee
• Keywords: Proteome; Keratin 6A

The human hair proteome was investigated using two-dimensional LC-MS/MS. Among the 343 identified proteins, 70 were detected in high relative abundance, including keratin intermediate filament largely extractable with denaturants. Over 300 proteins found to constitute insoluble complex formed by transglutaminase cross-linking. intracellular distribution of is wide from cytoplasm nucleus, mitochondria, ribosome, and plasma membrane. These results help rationalize ultrastructural features visible mature hair. Keratins several substrates for be posttranslationally modified methylation dimethylation. Evidence ubiquitination also obtained.