Proteomics Characterization of Mouse Kidney Peroxisomes by Tandem Mass Spectrometry and Protein Correlation Profiling

• DOI: 10.1074/mcp.m700169-mcp200
• Publication Date: 2007-09-04T00:12:56Z
• Authors: Sebastian Wiese; Thomas Gronemeyer; Rob Ofman; Markus Kunze; Cláudia P. Grou; José A. Almeida; Martin Eisenacher; Christian Stephan; Heiko Hayen; Lukas Schollenberger; Thomas Korosec; Hans R. Waterham; Wolfgang Schliebs; Ralf Erdmann; Johannes Berger; Helmut E. Meyer; Wilhelm Just; Jorge E. Azevedo; Ronald J.A. Wanders; Bettina Warscheid; Cláudia P. Grou
• Keywords: Proteome; Organelle

The peroxisome represents a ubiquitous single membrane-bound key organelle that executes various metabolic pathways such as fatty acid degradation by α- and β-oxidation, ether-phospholipid biosynthesis, metabolism of reactive oxygen species, detoxification glyoxylate in mammals. To fulfil this vast array functions, peroxisomes accommodate ∼50 different enzymes at least identified until now. Interest has been fueled the discovery group genetic diseases humans, which are caused either defect biogenesis or deficient activity distinct peroxisomal enzyme transporter. Although research greatly improved our understanding their role mammalian metabolism, deeper insight into biochemistry functions is required to expand knowledge low abundance but vital organelle. In work, we used classical subcellular fractionation combination with MS-based proteomics methodologies characterize proteome mouse kidney peroxisomes. We could identify virtually all known components involved biogenesis. Moreover through protein localization studies using quantitative MS screen combined statistical analyses, 15 new candidates. Of these, further investigated five candidates immunocytochemistry, confirmed As result joint effort, believe have compiled so far most comprehensive catalogue Peroxisomes small organelles present eukaryotic cells. They surrounded membrane harbor large set enables them execute an β-oxidation acids, mammals (1Wanders R.J. Waterham H.R. Biochemistry revisited.Annu. Rev. Biochem. 2006; 75: 295-332Crossref PubMed Scopus (692) Google Scholar). includes complex processes assembly, import matrix proteins, division mature These require concerted action machinery composed more than 20 so-called peroxins (2Heiland I. Erdmann R. Biogenesis Topogenesis proteins.FEBS J. 2005; 272: 2362-2372Crossref (120) Scholar, 3Brown L.A. Baker A. Peroxisome import.J. Cell. Mol. 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