Toward an Understanding of the Molecular Mechanism for Successful Blood Feeding by Coupling Proteomics Analysis with Pharmacological Testing of Horsefly Salivary Glands

• DOI: 10.1074/mcp.m700497-mcp200
• Publication Date: 2007-12-18T03:07:54Z
• Authors: Xueqing Xu; Hailong Yang; Dongying Ma; Jing Wu; Yipeng Wang; Yuzhu Song; Xu Wang; Yi Lu; Junxing Yang; Ren Lai
• Keywords: Lipopolysaccharides; Proteomics; 0301 basic medicine; DNA, Complementary; Platelet Aggregation; Diptera; Molecular Sequence Data; Hyaluronoglucosaminidase; Feeding Behavior; Chemical Fractionation; Salivary Glands; 3. Good health; Mice; 03 medical and health sciences; Animals; Insect Proteins; Metallothionein; Amino Acid Sequence; Cloning, Molecular; Peptides; Immunosuppressive Agents; Platelet Aggregation Inhibitors; Antimicrobial Cationic Peptides

Horseflies are economically important blood-feeding arthropods and also a nuisance for humans vectors filariasis. They rely heavily on the pharmacological properties of their saliva to get blood meal suppress immune reactions hosts. Little information is available antihemostatic substances in horsefly salivary glands; especially no suppressants have been reported. By proteomics or peptidomics coupling transcriptome analysis with testing, several families proteins peptides, which act mainly hemostatic system host, were identified characterized from 30,000 pairs glands Tabanus yao (Diptera, Tabanidae). are: (i) novel family inhibitors platelet aggregation including two members, possibly inhibit by mechanism membrane, (ii) immunosuppressant peptides 12 can interferon-gamma production increase interleukin-10 secretion, (iii) serine protease inhibitor 56 amino acid residues containing anticoagulant activity, (iv) (v) fibrinogenolytic (vi) three antimicrobial six (vii) hyaluronidase, (viii) vasodilator peptide, an isoform vasotab Hybomitra bimaculata, interestingly (ix) metallothioneins, first metallothioneins reported invertebrate glands. The current work will facilitate understanding molecular mechanisms ectoparasite-host relationship help identifying vaccine targets leading compounds.