Unrestrictive Identification of Multiple Post-translational Modifications from Tandem Mass Spectrometry Using an Error-tolerant Algorithm Based on an Extended Sequence Tag Approach

• DOI: 10.1074/mcp.m800101-mcp200
• Publication Date: 2008-08-14T00:25:01Z
• Authors: Seungjin Na; Jaeho Jeong; Heejin Park; Kong-Joo Lee; Eunok Paek
• Keywords: Identification; Sequence (biology); Posttranslational modification

Identification of post-translational modifications (PTMs) is important to understanding the biological functions proteins. MS/MS a useful tool identify PTMs. Most existing search tools are restricted take only few types PTMs as input. Here we describe new algorithm, called MOD(i) (pronounced "mod eye"), that rapidly searches for all known at once without limiting multitude modified sites in peptide. introduces notion tag chain, combination structure made from multiple sequence tags, effectively localizes regions within spectrum and overcomes de novo sequencing errors common tag-based approaches. showed its performance competence by identifying various analysis PTM-rich proteins such glyceraldehyde-3-phosphate dehydrogenase lens protein. We demonstrated innovatively manages computational complexity peptide, which may exist greater variety than usually expected. In addition, it suggested has great potential discover novel modifications.