Identification of Quantitative Trait Loci Underlying Proteome Variation in Human Lymphoblastoid Cells

• DOI: 10.1074/mcp.m900378-mcp200
• Publication Date: 2010-02-24T02:38:44Z
• Authors: Nikhil Garge; Huaqin Pan; Megan D. Rowland; Benjamin J. Cargile; Xinxin Zhang; Phillip C. Cooley; Grier P. Page; Maureen K. Bunger
• Keywords: International HapMap Project; Proteome; Human proteome project; Coding region

Population-based variability in protein expression patterns, especially humans, is often observed but poorly understood. Moreover, very little known about how interindividual genetic variation contributes to patterns. To begin address this, we describe elements of technical and biological variations contributing 544 proteins a population 24 individual human lymphoblastoid cell lines that have been extensively genotyped as part the International HapMap Project. We determined levels 10% were tightly correlated doubling rates. Using publicly available genotypes for these lines, applied association approach identify quantitative trait loci associated with variation. Results identified forms corresponding 15 which responsible >50% The located cis gene coding transcript 19 forms. Four non-synonymous single nucleotide polymorphisms resulted migration pattern changes two-dimensional gel. 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