Advanced glycation end products in Descemet’s membrane and their effect on corneal endothelial cell

• DOI: 10.1076/ceyr.23.6.469.6968
• Publication Date: 2003-02-20T14:08:21Z
• Authors: Yuichi Kaji; Shiro Amano; Tomohiko Usui; Kaori Suzuki; Sumiyoshi Tanaka; Tetsuro Oshika; Ryoji Nagai; Seiko Horiuchi
• Keywords: Descemet's membrane; Type IV collagen

Purpose. The purpose of this study was to evaluate the effect advanced glycation end products (AGEs) in Descemet's membrane on attachment and spreading corneal endothelial cells. Methods. An anti-AGEs monoclonal antibody (6D12), which recognizes a N e -carboxymethyl lysine (CML)-protein adduct as an epitope, used for immunohistochemistry enzyme-linked immunosorbent assay (ELISA). Fresh bovine incubated 4 weeks buffered solution with 500 mM glucose-6-phosphate (G-6-P). In membrane, immunohistochemical localization CML examined. Type I collagen-, type IV fibronectin-, or laminin-coated 96-well plates were glycated by G-6-P. amount determined ELISA using 6D12. Cultured cells seeded onto non-glycated extracellular matrix (ECM) allowed attach 3 hours. number surface area attached Results. Immunoreactivity detected containing Glycation fibronectin laminin decreased Aminoguanidine incubation mixture inhibited formation ECM components increased dose-dependent manner. Conclusions. AGE attenuated AGEs' may be responsible cell loss aging abnormalities diabetic patients