Novel tempeh (fermented soyabean) isoflavones inhibitin vivoangiogenesis in the chicken chorioallantoic membrane assay

• DOI: 10.1079/bjn20041330
• Publication Date: 2005-05-07T21:22:46Z
• Authors: Serafim Kiriakidis; Oliver Högemeier; Susanne Starcke; Frank Dombrowski; Jens Claus Hahne; Michael Pepper; Hem Chandra Jha; Nicolas Wernert
• Keywords: Chorioallantoic membrane

Anti-angiogenic strategies are emerging as an important tool for the treatment of cancer and inflammatory diseases. In present investigation we isolated several isoflavones from a tempeh (fermented soyabean) extract. The were identified 5,7,4′-trihydroxyisoflavone (genistein), 7,4′-dihydroxyisoflavone (daidzein), 6,7,4′-trihydroxyisoflavone (factor 2), 7,8,4′-trihydroxyisoflavone (7,8,4′-TriOH) 5,7,3′,4′-tetrahydroxyisoflavone (orobol). effects on angiogenesis these evaluated in chicken chorioallantoic membrane assay; their capacity to inhibit vascular endothelial growth factor-induced cell proliferation expression Ets 1 transcription factor, known be implicated regulation new blood vessel formation, also investigated. We found that all inhibited angiogenesis, albeit with different potencies. Compared negative controls, which slightly vivo by 6·30 %, genistein reduced angiogensis 75·09 followed orobol (67·96 %), factor 2 (56·77 daidzein (48·98 %) 7,8,4′-TriOH (24·42 %). These compounds proliferation, causing greatest inhibition at lower concentrations. expression, providing some insight into molecular mechanisms action. Furthermore, chemical structure suggests structure–activity relationship. Our findings suggest might added list low mass therapeutic agents angiogenesis.