Purification and characterization of acid phosphatase from larvae of the camel tick Hyalomma dromedarii : inhibition kinetics by sodium fluoride

• DOI: 10.1080/01647954.2023.2281509
• Publication Date: 2023-11-17T08:40:42Z
• Authors: Hassan M. M. Masoud; Mohamed S. Helmy; Doaa A. Darwish; Mohamed M. Abdel-...; Mahmoud A. Ibrahim
• Keywords: Enzyme Kinetics

Acid phosphatases (ACPs) perform a fundamental role in the digestion of egg proteins (vitellins), ensuring survival ticks by providing nutrients necessary for their oogenesis. Here, acid phosphatase activity is detected during embryogenesis camel tick Hyalomma dromedarii and reached its highest level larval stage. The larvae which was named TLACP homogeneously purified using CM cellulose cation exchange chromatography followed gel filtration on Sephacryl S-300 column. to 11 times purification folds 51.3% yield with specific 12.9 U mg−1 protein. molecular weight derived from both column SDS-PAGE as monomer protein 40 kDa. Km p-nitrophenyl phosphate (p-NPP) 1.25 mM Vmax 6.4 Umg−1. exhibited optimum enzymatic at pH 4.8. found highly p-NPP ATP, ADP, glucose-6-phosphate, naphthyl phosphate, phospho-enol-pyruvate, creatine AMP, GMP. ions Ca2+, Co2+, Mg2+, Ni2+, Zn2+ motivated while Mn2+, Cu2+, Fe2+ suppressed it. noncompetitively inhibited sodium fluoride Ki value 0.15 mM. Thus, presence embryonic cells classifies this enzyme an important target evolving potential vaccines further studies aiming find new strategies control ticks.