Differential variations of autophagy and apoptosis in permanent focal cerebral ischaemia rat model

• DOI: 10.1080/02699052.2017.1298005
• Publication Date: 2017-05-16T04:40:37Z
• Authors: Pengyue Zhang; Liqiang Yang; Hongyun He; Yihao Deng
• Keywords: Cerebral ischaemia

Objective: Autophagy and apoptosis coexist in stroke, but the relationship between effects complex is poorly understood. Herein, we investigated dynamic changes of autophagy at penumbra permanent cerebral ischaemia. Methods: Sprague-Dawley rat models were prepared by middle artery occlusion. The evaluated Western blotting immunofluorescence with LC3-II cleaved caspase-3, respectively. neurological deficit score infarct volume assessed. Results: results showed that expressions caspase-3 gradually increased from 1 to 5 hours, reached maximum hours after stroke. After that, expression was significantly declined, only mildly reduced 6 3 days. Surprisingly, 4 days abruptly again, considerably continuously decreased. severity accordance increase expansion. Conclusions: Our simultaneously activated within 12 Four later, increased, while decreased, implying there might be a transition subacute phase