The potential role of differentially expressed tRNA-derived fragments in high glucose-induced podocytes

• DOI: 10.1080/0886022x.2024.2318413
• Publication Date: 2024-02-19T06:17:48Z
• Authors: Zhenxing Zhang; Yunyang Qiao; Jialing Ji; Chan Huang; Huimin Shi; Weihua Gan; Aiqing Zhang
• Keywords: KEGG; Nephrin; Podocin

The prevalence of diabetic kidney disease (DKD) is increasing annually. Damage to and loss podocytes occur early in DKD. tRNA-derived fragments (tRFs), originating from tRNA precursors or mature tRNAs, are associated with various illnesses. In this study, tRFs were identified, their roles podocyte injury induced by high-glucose (HG) treatment explored. High-throughput sequencing treated HG was performed identify differentially expressed tRFs. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses performed. expression levels nephrin, podocin, desmin measured after overexpression tRF-1:24-Glu-CTC-1-M2 (tRF-1:24) concomitant treatment. A total 647 89 (|log2FC| ≥ 0.585; p ≤ .05) identified the group, which 53 downregulated 36 upregulated. 10 highest differential detected real-time quantitative polymerase chain reaction (RT-qPCR), these results consistent results. GO analysis revealed that biological process, cellular component, molecular function terms most enriched processes, anatomical entities, binding. KEGG pathway may be involved signaling pathways related growth hormones, phospholipase D, regulation stem cell pluripotency, T-/B-cell receptors. Overexpression tRF-1:24, one tRFs, attenuated HG. Thus, might potential biomarkers for