Characterization of Essential Oil and Wastewater from Thymus nummularius M. Bieb. and Micromorphological Examination of Glandular Trichomes

• DOI: 10.1080/0972060x.2022.2107403
• Publication Date: 2022-08-09T03:17:38Z
• Authors: Yusuf Can Gerçek; Sinan Bayram; Saffet Çelik; Deniz Canlı; Muhammed Halid Ma...; Kadir Boztas; Fatma Nur Bastürk; Seran Kırkıncı; Yeter Yesil; Taylan Kösesakal; Gül Cevahir Öz; Nesrin Ecem Bayram
• Keywords: Thymol; ABTS

This study aimed to determine the volatile profile by GC-MS and antimicrobial activity of essential oil isolated from Thymus nummularius using microwave-assisted hydrodistillation method morphologically characterize glandular trichomes in leaf samples. Obtained oil, as well wastewater obtained during isolation, were chemically (polyphenolic compounds, organic acids, total phenolic content, flavonoid content antioxidant activity) characterized. As a result analysis different ratios components identified, predominantly thymol (38.91%), linalool (13.12%) geraniol (6.51%). The results showed that T. has broad-spectrum inhibitory effect against all target microorganisms (bacteria yeast-like fungi). Screening 32 polyphenolic compounds 52 acids was performed LC-MS/MS major compound acid determined caffeic (97316.85 µg/kg) malic (6898.88 mg/kg), respectively. While 35.91 mg GA/g 34.91 QE/g, respectively, CUPRAC, CERAC ABTS tests 86.28 TE/g, 72.85 TE/g 47.88 TE/g. These support nummularius, an important production area Turkey, can be used industrial areas due its rich bioactive content. In addition, it said is also considered valuable source substances.