Marine diatom assemblages of the Nosy Be Island coasts, NW Madagascar: species composition and biodiversity using molecular and morphological taxonomy

• DOI: 10.1080/14772000.2019.1696420
• Publication Date: 2019-12-23T13:54:25Z
• Authors: Adrian Kryk; MaŁgorzata BA˛k; Ewa GÓrecka; Catherine Riaux-G...; John Bemiasa; Etienne Bemanaja; Chunlian Li; PrzemysŁaw DA˛Bek; Andrzej Witkowski
• Keywords: Navicula; Nitzschia; DNA Barcoding; Endemism

Madagascar is an isolated oceanic island characterized by high degrees of endemism at all taxonomic levels. The waters surrounding are still poorly studied for benthic marine diatom assemblages. aim our study was to analyse the biodiversity and molecular phylogeny assemblages Nosy Be archipelago in terms their ubiquity/rarity. A total 65 samples from 14 sampling sites were collected Tanikely islands June/July 2014. Diatom identification, based on light electron microscopic examination, revealed 332 taxa. From those, ∼90 strains successfully cultured. So far, DNA has been extracted one chloroplast gene (rbcL) sequenced 21 order reconstruct single phylogeny. Taxa selected sequencing preliminary genera considered common planktonic forms littoral: Bellerochea, Melosira, Paralia, Triceratium, Navicula, Hippodonta, Auricula, Nitzschia, Cocconeis, some araphids. For each site, Shannon Simpson indices calculated. biodiversity. most abundant species Cocconeis scutellum. Whereas cultured taxa seem represent cosmopolitan or zonal forms: e.g., Bellerochea malleus, Triceratium dubium, Melosira lineata, Paralia crawfordii, Nitzschia inconspicua, culture have not observed earlier works use markers. dominant generally those with broad geographic distribution global/Indo-Pacific affinities. Amongst taxa, only 35% identified level. Many generic level may turn out new science after further SEM examination sequencing.