Circular RNA SMARCA5 functions as an anti-tumor candidate in colon cancer by sponging microRNA-552

• DOI: 10.1080/15384101.2021.1899519
• Publication Date: 2021-03-22T11:09:33Z
• Authors: Shiwei Yang; Shanyu Gao; Tongming Liu; Junning Liu; Xia Zheng; Zhi Li
• Keywords: Adenosine Triphosphatases; Mice, Inbred BALB C; 0303 health sciences; Chromosomal Proteins, Non-Histone; Mice, Nude; 3. Good health; Mice; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Colonic Neoplasms; Biomarkers, Tumor; Animals; Humans

It was reported that circular RNA (circRNA) circSMARCA5, as a tumor-related molecule, could modulate development of cancers, including prostatic cancer and cervical cancer. Nevertheless, the essential function of circSMARCA5 in colon cancer has not yet been confirmed. We aimed to investigate the role of circSMARCA5 in colon cancer. CircSMARCA5 expression in tumor cells was detected using RT-qPCR. CCK-8, colony formation, flow cytometry and Transwell assays evaluated the influences of circSMARCA5 in colon cancer cells. RT-qPCR, prediction database and luciferase report assay were accomplished for revealing the correlation between circSMARCA5 and miR-552. After transfection with miR-552 mimic, colon cancer cell behaviors were re-evaluated. Wnt and YAP1 pathways were explored by western blot. Our data presented that circSMARCA5 was under-expressed in colon cancer tissues. Transfection with overexpressing circSMARCA5 plasmid restrained growth, migration and invasion of colon cancer cells. Besides, circSMARCA5 directly sponged to miR-552 and miR-552 up-regulation offset the effects of circSMARCA5 on SW480 and SW620 cells. Furthermore, circSMARCA5 inactivated Wnt and YAP1 pathways by inhibiting miR-552. Anti-tumor role of sircSMARCA5 was showed in colon cancer cells as sponging miR-552 and blocking Wnt and YAP1 pathways.