MicroRNA analysis of Natural Killer cell-derived exosomes: the microRNA let-7b-5p is enriched in exosomes and participates in their anti-tumor effects against pancreatic cancer cells

• DOI: 10.1080/2162402x.2023.2221081
• Publication Date: 2023-06-08T04:55:45Z
• Authors: Anna Laura Di Pace; Andrea Pelosi; Piera Filomena Fiore; Nicola Tumino; Francesca Besi; Linda Quatrini; Silvia Santopolo; Paola Vacca; Lorenzo Moretta
• Keywords: microRNA; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; exosomes; RC581-607; Exosomes; 3. Good health; Pancreatic Neoplasms; Killer Cells, Natural; MicroRNAs; gene expression profiling; Natural killer cells; tumor microenvironment; Humans; Immunologic diseases. Allergy; Child; RC254-282; Original Research

Natural Killer (NK) cells are important components of the immune system in defense against tumor growth and metastasis. They release exosomes containing proteins nucleic acids, including microRNAs (miRNAs). NK-derived play a role anti-tumor NK cell function since they able to recognize kill cancer cells. However, involvement exosomal miRNAs is poorly understood. In this study, we explored miRNA content by microarray as compared their cellular counterparts. The expression selected lytic potential childhood B acute lymphoblastic leukemia after co-cultures with pancreatic were also evaluated. We identified small subset miRNAs, miR-16-5p, miR-342-3p, miR-24-3p, miR-92a-3p let-7b-5p that highly expressed exosomes. Moreover, provide evidence efficiently increase induce inhibition proliferation targeting cycle regulator CDK6. Let-7b-5p transfer could represent novel mechanism which counteract growth. both cytolytic activity reduced upon co-culture Alteration cargo exosomes, together cytotoxic activity, another strategy exerted evade response. Our study provides new information on molecular mechanisms used exert anti-tumor-activity offers clues integrate treatments