Microtubule-associated protein 2 within axons of spinal motor neurons: associations with microtubules and neurofilaments in normal and beta,beta'-iminodipropionitrile-treated axons.

• DOI: 10.1083/jcb.100.1.74
• Publication Date: 2004-05-15T00:18:20Z
• Authors: S C Papasozomenos; L I Binder; P K Bender; M R Payne
• Keywords: Neurofilament; Dorsal root ganglion; GDF7; Peripheral Nervous System; Axoplasmic transport

We have examined the distribution of microtubule-associated protein 2 (MAP2) in lumbar segment spinal cord, ventral and dorsal roots, root ganglia control beta,beta'-iminodipropionitrile-treated rats. The peroxidase-antiperoxidase technique was used for light electron microscopic immunohistochemical studies with two monoclonal antibodies directed against different epitopes Chinese hamster brain MAP2, designated AP9 AP13. MAP2 immunoreactivity present axons motor neurons, but not detected white matter tracts cord majority root. A gradient staining intensity among dendrites, cell bodies, neurons present, dendrites most intensely least. While bodies all were positive, variably stained. labeled ganglion cells up to their bifurcation; beyond this point, while only occasional central processes roots weakly stained, peripheral nerves positive. beta,beta'-Iminodipropionitrile produced segregation microtubules membranous organelles from neurofilaments nervous system portion accumulation axons. both anti-MAP2 hybridoma co-localized portion, one axons, other antibody did stain region axon which contained microtubules. These findings suggest that (a) is albeit a lower concentration or form than (b) interacts neurofilaments.