Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells.

• DOI: 10.1083/jcb.107.1.321
• Publication Date: 2004-05-15T00:18:20Z
• Authors: M L Dustin; T A Springer
• Keywords: Lymphocyte homing receptor; Cell–cell interaction; Intercellular adhesion molecule; Lymphoblast

Intercellular adhesion molecule-1 (ICAM-1) on the surface of cultured umbilical vein and saphenous endothelial cells was upregulated between 2.5- 40-fold by rIL-1, rTNF, LPS rIFN gamma corresponding to up 5 X 10(6) sites/cell. Endothelial cell ICAM-1 a single band 90 kD in SDS-PAGE. Purified reconstituted into liposomes bound plastic an excellent substrate for both JY B lymphoblastoid T lymphoblast adhesion. Adhesion planar membranes blocked completely monoclonal antibodies lymphocyte function associated antigen-1 (LFA-1) or ICAM-1. artificial most sensitive density within physiological range found resting stimulated cells. cells, normal genetically LFA-1 deficient lymphoblasts peripheral blood lymphocytes monolayers also assayed. In summary, dependent (60-90% total adhesion) LFA-1-independent basal observed use pathways different interacting pairs increased monokine lipopolysaccharide stimulation The LFA-1-dependent could be further subdivided LFA-1/ICAM-1-dependent component which cytokines LFA-1-dependent, ICAM-1-independent did not appear affected cytokines. We conclude that is regulated ligand lymphocyte-endothelial adhesion, but at least two other major exist.