Identification of peroxisomal targeting signals located at the carboxy terminus of four peroxisomal proteins.

• DOI: 10.1083/jcb.107.3.897
• Publication Date: 2004-05-15T00:18:20Z
• Authors: S J Gould; G A Keller; S Subramani
• Keywords: Peroxisomal targeting signal; Formate dehydrogenase; Microbody; Tripeptide

As part of an effort to understand how proteins are imported into the peroxisome, we have sought identify peroxisomal targeting signals in four unrelated proteins: human catalase, rat hydratase:dehydrogenase, pig D-amino acid oxidase, and acyl-CoA oxidase. Using gene fusion experiments, identified a region each protein that can direct heterologous peroxisomes. In case, signal is contained at or near carboxy terminus protein. For located within COOH-terminal 27 amino acids carboxy-terminal 15, 14, 15 acids, respectively. A tripeptide sequence Ser-Lys/His-Leu present these as well firefly luciferase (Gould, S.J., G.-A. Keller, S. Subramani. 1987. J. Cell Biol. 105:2923-2931). When hydratase:dehydrogenase mutated so Ser-Lys-Leu converted Ser-Asn-Leu, it no longer We suggest this essential element least one class signals.