Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.

• DOI: 10.1083/jcb.123.2.431
• Publication Date: 2004-05-15T00:22:22Z
• Authors: K Ainger; D Avossa; F Morgan; S J Hill; C Barry; E Barbarese; J H Carson
• Keywords: Granule (geology)

We have studied transport and localization of MBP mRNA in oligodendrocytes culture by microinjecting labeled into living cells analyzing the intracellular distribution injected RNA confocal microscopy. Injected initially appears dispersed perikaryon. Within minutes, forms granules which, case mRNA, are transported down processes to periphery cell where again becomes dispersed. In situ hybridization shows that endogenous also as perikaryon peripheral membranes. The not released extraction with non-ionic detergent, indicating they associated cytoskeletal matrix. Three dimensional visualization indicates often aligned tracks along microtubules traversing cytoplasm processes. Several distinct patterns granule movement observed. Granules undergo sustained directional a velocity approximately 0.2 micron/s. at branch points oscillatory motion mean displacement 0.1 circulate randomly 1 results discussed terms multi-step pathway for oligodendrocytes. This work represents first characterization cells, description role cells.