Distinct cell surface ligands mediate T lymphocyte attachment and rolling on P and E selectin under physiological flow.

• DOI: 10.1083/jcb.127.5.1485
• Publication Date: 2004-05-15T00:22:22Z
• Authors: R Alon; H Rossiter; X Wang; T A Springer; T S Kupper
• Keywords: E-selectin; High endothelial venules; Extravasation; L-selectin; Leukocyte extravasation; P-selectin

Memory T lymphocytes extravasate at sites of inflammation, but the mechanisms employed by these cells to initiate contact and tethering with endothelium are incompletely understood. An important part leukocyte extravasation is initiation rolling adhesions on endothelial selectins; such events have been studied in monocytes neutrophils not lymphocytes. In this study, potential adhere roll selectins vitro was investigated. We demonstrate that can form tethers P selectin E under physiologic flow conditions. Tethering independent cell-surface cutaneous lymphocyte antigen (CLA) expression, which correlated strictly capacity selectin. cell abolished selective removal surface sialomucins a P. haemolytica O-glycoprotease, while expression unaffected. A sialomucin molecule identical or closely related glycoprotein ligand-1 (PSGL-1), major ligand HL-60 cells, appears be for does appear support turn, ligands do associated sialomucins. These data presence structurally distinct provide evidence both coexpressed single cell, mediate respective mutually exclusive fashion.