A potential role for tetranectin in mineralization during osteogenesis.

• DOI: 10.1083/jcb.127.6.1767
• Publication Date: 2004-05-15T00:22:22Z
• Authors: U M Wewer; K Ibaraki; P Schjørring; M E Durkin; M F Young; R Albrechtsen
• Keywords: Bone sialoprotein; Immunostaining

Tetranectin is a protein shared by the blood and extracellular matrix. composed of four identical, noncovalently bound polypeptides each with molecular mass approximately 21 kD. There some evidence that tetranectin may be involved in fibrinolysis proteolysis during tissue remodeling, but its precise biological function not known. enriched cartilage shark, gene expression pattern mammalian skeletal system has been determined. In present study we have examined putative osteogenesis. newborn mouse, strong immunoreactivity was found newly formed woven bone around anlage future marrow along periosteum forming cortex. No proliferating hypertrophic or surrounding muscle. Using an vitro mineralizing system, osteoblastic cells at different times their growth differentiation. mRNA appeared cultured parallel mineralization, similar to sialoprotein, which regarded as one late differentiation markers. To explore role osteogenesis established stably transfected cell lines (PC12-tet) overexpressing recombinant evidenced Northern Western blot analysis immunoprecipitation. Both control PC12 PC12-tet injected into nude mice produced tumors containing material, von Kossa staining for calcium immunostaining sialoprotein alkaline phosphatase antiserum. Nude from contained fivefold more material than those untransfected line vector no insert (Mann Whitney rank sum test, p < 0.01), supporting notion play important direct and/or indirect conclusion, potential matrix expressed time space coincident mineralization vivo vitro.