Integrin-mediated Activation of Focal Adhesion Kinase Is Required for Signaling to Jun NH2-terminal Kinase and Progression through the G1 Phase of the Cell Cycle

• DOI: 10.1083/jcb.145.7.1461
• Publication Date: 2002-07-26T16:45:41Z
• Authors: Maja Oktay; Kishore K. Wary; Michael Dans; Raymond B. Birge; Filippo G. Giancotti
• Keywords: PTK2; Integrin-linked kinase

The extracellular matrix exerts a stringent control on the proliferation of normal cells, suggesting existence mitogenic signaling pathway activated by integrins, but not significantly growth factor receptors. Herein, we provide evidence that integrins cause significant and protracted activation Jun NH2-terminal kinase (JNK), while several factors more modest or no this enzyme. Integrin-mediated stimulation JNK required association focal adhesion (FAK) with Src p130CAS, phosphorylation subsequently, recruitment Crk. Ras PI-3K were required. FAK–JNK was necessary for proper progression through G1 phase cell cycle. These findings establish role FAK in both cycle, identify physiological stimulus is consistent transformation.