The Telomerase/Vault-Associated Protein Tep1 Is Required for Vault RNA Stability and Its Association with the Vault Particle

• DOI: 10.1083/jcb.152.1.157
• Publication Date: 2002-07-26T16:46:23Z
• Authors: Valerie A. Kickho...; Yie Liu; Lawrence B. Kong; Bryan E. Snow; Phoebe L. Stewart; Lea Harrington; Leonard H. Rome
• Keywords: Vault (architecture); Ribonucleoprotein particle

Vaults and telomerase are ribonucleoprotein (RNP) particles that share a common protein subunit, TEP1. Although its role in either complex has not yet been defined, TEP1 shown to interact with the mouse RNA several of human vault RNAs yeast three-hybrid assay. An mTep1(-/-) was previously generated which resulted no apparent change telomere length or activity six generations mTep1-deficient mice. Here we show levels association RNP also unaffected vaults purified from livers mice appear structurally intact by both negative stain cryoelectron microscopy, three-dimensional reconstruction revealed less density cap than observed for rat vault. Furthermore, absence completely disrupted stable particle decrease stability RNA. Therefore, have uncovered novel vivo as an integral important stabilization recruitment particle.