Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)–related protein Sli15 during chromosome segregation

• DOI: 10.1083/jcb.200105029
• Publication Date: 2002-07-26T16:46:23Z
• Authors: Jung-seog Kang; Iain M. Cheeseman; George Kallstrom; Soundarapandian V...; Georjana Barnes; Clarence S.M. Chan
• Keywords: Aurora B kinase; Spindle pole body

We have shown previously that Ipl1 and Sli15 are required for chromosome segregation in Saccharomyces cerevisiae. associates directly with the protein kinase these two proteins colocalize to mitotic spindle. show here stimulates vitro, likely vivo, activity of Ipl1, facilitates association The Ipl1-binding -stimulating activities both reside within a region containing homology metazoan inner centromere (INCENP). also bind Dam1, microtubule-binding spindle integrity kinetochore function. Dam1 most physiological targets since can phosphorylate efficiently vivo phosphorylation is reduced ipl1 mutants. Some dam1 mutations exacerbate phenotype sli15 mutants, thus providing evidence interactions Ipl1–Sli15 functionally important vivo. Similar each microtubules they associated yeast centromeric DNA Given their dual kinetochores, Sli15, may play crucial roles regulating chromosome–spindle or movement kinetochores along microtubules.