Distinct molecular and cellular contributions to stabilizing selectin-mediated rolling under flow

• DOI: 10.1083/jcb.200204041
• Publication Date: 2002-09-18T18:04:15Z
• Authors: Tadayuki Yago; Anne Leppänen; Haiying Qiu; Warren D. Marcus; Matthias U. Nollert; Cheng Zhu; Richard D. Cummings; Rodger P. McEver
• Keywords: Glycocalyx; P-selectin; K562 cells; L-selectin

Leukocytes roll on selectins at nearly constant velocities over a wide range of wall shear stresses. Ligand-coupled microspheres faster and detach quickly as stress is increased. To examine whether the superior performance leukocytes reflects molecular features native ligands or cellular properties that favor selectin-mediated rolling, we coupled structurally defined selectin to K562 cells compared their rolling P-selectin. Microspheres bearing soluble P-selectin glycoprotein ligand (sPSGL)-1 2-glycosulfopeptide (GSP)-6, GSP modeled after NH2-terminal P-selectin–binding region PSGL-1, rolled equivalently but unstably displaying randomly 2-GSP-6 also unstably. In contrast, sPSGL-1 targeted membrane-distal presumed glycocalyx more like leukocytes: steps were uniform resistant, tended plateau was treated with paraformaldehyde methyl-β-cyclodextrin before coupling less deformable microspheres. Cells cytochalasin D deformable, further resisted detachment, slowly despite increases in stress. Thus, stable, shear-resistant requires optimize selectin–ligand interactions.