Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice

• DOI: 10.1083/jcb.200412022
• Publication Date: 2005-05-03T00:14:07Z
• Authors: Masaaki Komatsu; Satoshi Waguri; Takashi Ueno; Junichi Iwata; Shigeo Murata; Isei Tanida; Junji Ezaki; Noboru Mizushima; Yoshinori Ohsumi; Yasuo Uchiyama; Eiki Kominami; Keiji Tanaka; Tomoki Chiba
• Keywords: Organelle; Autophagosome; BAG3

Autophagy is a membrane-trafficking mechanism that delivers cytoplasmic constituents into the lysosome/vacuole for bulk protein degradation. This involved in preservation of nutrients under starvation condition as well normal turnover component. Aberrant autophagy has been reported several neurodegenerative disorders, hepatitis, and myopathies. Here, we generated conditional knockout mice Atg7, an essential gene yeast. Atg7 was ATG conjugation systems autophagosome formation, amino acid supply neonates, starvation-induced degradation proteins organelles mice. Furthermore, deficiency led to multiple cellular abnormalities, such appearance concentric membranous structure deformed mitochondria, accumulation ubiquitin-positive aggregates. Our results indicate important role response quality control quiescent cells.