WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells
Formins
MDia1
Wiskott–Aldrich syndrome protein
DOI:
10.1083/jcb.200708123
Publication Date:
2008-03-24T17:34:13Z
AUTHORS (12)
ABSTRACT
We examined the role of actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion response to epidermal growth factor (EGF), a key regulator carcinoma invasion. found that knockdown (KD) suppresses lamellipod formation increases filopod formation, whereas N-WASP KD has no effect. However, simultaneous both proteins results large jagged protrusions with lamellar properties increased formation. This suggests another activity is at work cells EGF. A mammalian Diaphanous-related formin, mDia1, localizes double cells. Constitutively active mDia1 recapitulated phenotype, inhibition blocked these protrusions. Increased RhoA activity, which stimulates nucleation, was observed N-WASP/WAVE2 shown be required for phenotype. These data show coordinate regulation between WASP family mDia controls balance lamellipodial activity.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (54)
CITATIONS (120)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....