WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells

Formins MDia1 Wiskott–Aldrich syndrome protein
DOI: 10.1083/jcb.200708123 Publication Date: 2008-03-24T17:34:13Z
ABSTRACT
We examined the role of actin nucleation promoters neural Wiskott-Aldrich syndrome protein (N-WASP) and WAVE2 in cell protrusion response to epidermal growth factor (EGF), a key regulator carcinoma invasion. found that knockdown (KD) suppresses lamellipod formation increases filopod formation, whereas N-WASP KD has no effect. However, simultaneous both proteins results large jagged protrusions with lamellar properties increased formation. This suggests another activity is at work cells EGF. A mammalian Diaphanous-related formin, mDia1, localizes double cells. Constitutively active mDia1 recapitulated phenotype, inhibition blocked these protrusions. Increased RhoA activity, which stimulates nucleation, was observed N-WASP/WAVE2 shown be required for phenotype. These data show coordinate regulation between WASP family mDia controls balance lamellipodial activity.
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