The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step

570 0303 health sciences Glucose Transporter Type 4 1.1 Normal biological development and functioning 610 Biological Transport Endosomes Biological Sciences Medical and Health Sciences Cell Line Myoblasts Mice 03 medical and health sciences Underpinning research Clathrin Heavy Chains Animals Humans Research Articles Developmental Biology trans-Golgi Network
DOI: 10.1083/jcb.200908057 Publication Date: 2010-01-11T19:05:44Z
ABSTRACT
Clathrin heavy chain 22 (CHC22) is an isoform of the well-characterized CHC17 clathrin heavy chain, a coat component of vesicles that mediate endocytosis and organelle biogenesis. CHC22 has a distinct role from CHC17 in trafficking glucose transporter 4 (GLUT4) in skeletal muscle and fat, though its transfection into HEK293 cells suggests functional redundancy. Here, we show that CHC22 is eightfold less abundant than CHC17 in muscle, other cell types have variably lower amounts of CHC22, and endogenous CHC22 and CHC17 function independently in nonmuscle and muscle cells. CHC22 was required for retrograde trafficking of certain cargo molecules from endosomes to the trans-Golgi network (TGN), defining a novel endosomal-sorting step distinguishable from that mediated by CHC17 and retromer. In muscle cells, depletion of syntaxin 10 as well as CHC22 affected GLUT4 targeting, establishing retrograde endosome–TGN transport as critical for GLUT4 trafficking. Like CHC22, syntaxin 10 is not expressed in mice but is present in humans and other vertebrates, implicating two species-restricted endosomal traffic proteins in GLUT4 transport.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (62)
CITATIONS (54)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....