Phosphoinositide 3-kinase signaling pathway mediated by p110α regulates invadopodia formation

• DOI: 10.1083/jcb.201009126
• Publication Date: 2011-06-28T03:11:55Z
• Authors: Hideki Yamaguchi; Shuhei Yoshida; Emi Muroi; Nachi Yoshida; Masahiro Kawamura; Zen Kouchi; Yoshikazu Nakamura; Ryuichi Sakai; Kiyoko Fukami
• Keywords: Invadopodia; Phosphoinositide 3-kinase; Proto-Oncogene Proteins c-akt; P110α

Invadopodia are extracellular matrix-degrading protrusions formed by invasive cancer cells that thought to function in invasion. Although many invadopodia components have been identified, signaling pathways link stimuli formation remain largely unknown. We investigate the role of phosphoinositide 3-kinase (PI3K) during formation. find human breast cells, both and degradation a gelatin matrix were blocked treatment with PI3K inhibitors or sequestration D-3 phosphoinositides. Functional analyses revealed among family proteins, class I catalytic subunit p110α, frequently mutated gene product cancers, was selectively involved The expression p110α cancerous mutations promoted invadopodia-mediated activity. Furthermore, knockdown inhibition PDK1 Akt, downstream effectors signaling, suppressed induced mutants. These data suggest via regulates invasion cells.