Ipl1/Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC–spindle interaction to ensure proper microtubule dynamics

• DOI: 10.1083/jcb.201009137
• Publication Date: 2011-07-05T02:20:06Z
• Authors: Yuko Nakajima; Anthony Cormier; Randall G. Tyers; Adrianne Pigula; Yutian Peng; David G. Drubin; Georjana Barnes
• Keywords: Spindle checkpoint; Microtubule nucleation; Microtubule polymerization; Aurora B kinase; Microtubule organizing center; Spindle pole body

Dynamic microtubules facilitate chromosome arrangement before anaphase, whereas during anaphase microtubule stability assists separation. Changes in dynamics at the metaphase–anaphase transition are regulated by Cdk1. Cdk1-mediated phosphorylation of Sli15/INCENP promotes preanaphase preventing chromosomal passenger complex (CPC; Sli15/INCENP, Bir1/Survivin, Nbl1/Borealin, Ipl1/Aurora) association with spindles. However, whether Cdk1 has sole control over dynamics, and how CPC–microtubule influences behavior, unclear. Here, we show that Ipl1/Aurora-dependent modulates CPC binding to spindle central until late facilitating spatiotemporal required for proper metaphase centromere positioning elongation. Decreased Ipl1-dependent Sli15 drives direct microtubules, revealing dynamics. We propose Ipl1/Aurora cooperatively modulate controls function excluding from regions engaged polymerization.