p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis

• DOI: 10.1083/jcb.201101148
• Publication Date: 2011-09-19T18:11:20Z
• Authors: Michael Y. Fessing; Andrei N. Mardaryev; Michal R. Gdula; Andrey A. Sharov; Tatyana Y. Sharova; Valentina Rapisarda; Konstantin B. Gordon; Anna D. Smorodchenko; Krzysztof Poterlo...; Giustina Ferone; Yoshinori Kohwi; Caterina Missero; Terumi Kohwi-Shig...; Vladimir A. Botch...
• Keywords: Chromatin immunoprecipitation; Epidermis (zoology)

During development, multipotent progenitor cells establish tissue-specific programs of gene expression. In this paper, we show that p63 transcription factor, a master regulator epidermal morphogenesis, executes its function in part by directly regulating expression the genome organizer Satb1 cells. binds to proximal regulatory region gene, and ablation results marked reduction levels epidermis. Satb1−/− mice impaired morphology. Satb1-null epidermis, chromatin architecture differentiation complex locus containing genes associated with is altered primarily at central domain, where binding was confirmed immunoprecipitation–on-chip analysis. Furthermore, within domain fail be properly activated upon terminal differentiation. p63+/− skin explants treated small interfering ribonucleic acid partially restored phenotype p63-deficient mice. These data provide novel mechanism which Satb1, direct downstream target p63, contributes morphogenesis via establishing organization