Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes

• DOI: 10.1083/jcb.201111091
• Publication Date: 2012-08-20T15:34:13Z
• Authors: Yu Chen; Yan Wang; Jinzhong Zhang; Yongqiang Deng; Li Jiang; Eli Song; Xufeng S. Wu; John A. Hammer; Tao Xu; Jennifer Lippinco...
• Keywords: GLUT4; Rab; Vesicle fusion; Transport protein

Rab proteins are important regulators of insulin-stimulated GLUT4 translocation to the plasma membrane (PM), but precise steps in trafficking modulated by particular remain unclear. Here, we systematically investigate involvement trafficking, focusing on directly mediating storage vesicle (GSV) delivery PM. Using dual-color total internal reflection fluorescence (TIRF) microscopy and an insulin-responsive aminopeptidase (IRAP)-pHluorin fusion assay, demonstrated that Rab10 facilitated GSV docking at Rab14 mediated PM via endosomal compartments containing transferrin receptor (TfR), whereas Rab4A, Rab4B, Rab8A recycled through system. Myosin-Va associated with GSVs interacting Rab10, positioning peripherally recruited for ultimate fusion. Thus, multiple regulate GLUT4, coordinating myosin-Va mediate final