Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes
GLUT4
Rab
Vesicle fusion
Transport protein
DOI:
10.1083/jcb.201111091
Publication Date:
2012-08-20T15:34:13Z
AUTHORS (10)
ABSTRACT
Rab proteins are important regulators of insulin-stimulated GLUT4 translocation to the plasma membrane (PM), but precise steps in trafficking modulated by particular remain unclear. Here, we systematically investigate involvement trafficking, focusing on directly mediating storage vesicle (GSV) delivery PM. Using dual-color total internal reflection fluorescence (TIRF) microscopy and an insulin-responsive aminopeptidase (IRAP)-pHluorin fusion assay, demonstrated that Rab10 facilitated GSV docking at Rab14 mediated PM via endosomal compartments containing transferrin receptor (TfR), whereas Rab4A, Rab4B, Rab8A recycled through system. Myosin-Va associated with GSVs interacting Rab10, positioning peripherally recruited for ultimate fusion. Thus, multiple regulate GLUT4, coordinating myosin-Va mediate final
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (42)
CITATIONS (144)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....